The specialist for automated sample preparation
LCTech GmbH has developed a new procedure of sample loading, the bidirectional SPE (solid phase extraction). Thus, even very difficult matrices with smallest sample volumes, like brain, can be processed automatically without supervision over 24 hours and 7 days per week.
Using the bidirectional SPE
the sample is aspirated through the LUER tip into the column. It passes the sorbent from bottom to top and is released directly afterwards into a vial from top to bottom whereas the sample passes the sorbent for the second time.
With this kind of sample loading and releasing the sample never enters the robotic system and a contamination of one sample to the next one can be excluded. Unwanted matrix effects are minimised. The elution of the analytes is possible with only a minimal amount of solvents. The original applied sample is collected in a vial next to the sample vial and is available for further processing if needed.
A cooperation with Dr. T. Stimpfl
of the Medical University of Vienna has shown that the procedure of bidirectional SPE is especially suitable for acidic, neutral or basic analytes in brain tissue or similar matrices. Lipids and proteins make the automated handling of these matrices particularly difficult, as the samples can contaminate the system due to their characteristics.
The risk of cross-contamination is very high, if the samples are loaded with a standard procedure and can only be prevented with intensive rinsing. However with the procedure of bidirectional SPE the user can have the samples processed around the clock, safe and reliable.
Press release LCTech v. 5.2.2016
Sample Loading for the Automated Sample Preparation
You can see the FREESTYLE robotic system with the new bidirectional SPE procedure for yourself at
in Munich, Germany,
in hall A2 booth 400